Understanding the Molecular Basis for the Inhibition of the Alzheimer's Aβ-Peptide Oligomerization by Human Serum Albumin Using Saturation Transfer Difference and Off-Resonance Relaxation NMR Spectroscopy

Human serum albumin (HSA) inhibits the formation of amyloid beta-peptide (Abeta) fibrils in human plasma. However, currently it is not known how HSA affects the formation of the highly toxic soluble diffusible oligomers that occur in the initial stages of Abeta fibrillization. We have therefore investigated by solution NMR the interaction of HSA with the Abeta(12-28) peptide, which has been previously shown to provide a reliable and stable …