Journal article
The Auto-Inhibitory Role of the EPAC Hinge Helix as Mapped by NMR
Abstract
The cyclic-AMP binding domain (CBD) is the central regulatory unit of exchange proteins activated by cAMP (EPAC). The CBD maintains EPAC in a state of auto-inhibition in the absence of the allosteric effector, cAMP. When cAMP binds to the CBD such auto-inhibition is released, leading to EPAC activation. It has been shown that a key feature of such cAMP-dependent activation process is the partial destabilization of a structurally conserved hinge …
Authors
Selvaratnam R; Mazhab-Jafari MT; Das R; Melacini G
Journal
PLOS ONE, Vol. 7, No. 11,
Publisher
Public Library of Science (PLoS)
Publication Date
2012
DOI
10.1371/journal.pone.0048707
ISSN
1932-6203