Journal article
Betaine aldehyde dehydrogenase from spinach leaves: Purification, in vitro translation of the mRNA, and regulation by salinity
Abstract
Spinach (Spinacia oleracea L.) leaves contain a nuclear-encoded chloroplastic betaine aldehyde dehydrogenase (EC 1.2.1.8) which is induced several-fold by salinization. Betaine aldehyde dehydrogenase was purified 2400-fold to homogeneity with an overall yield of 14%. The procedure included fractional precipitation with ammonium sulfate, followed by ion-exchange, hydrophobic interaction, and hydroxyapatite chromatography in open columns, and …
Authors
Weretilnyk EA; Hanson AD
Journal
Archives of Biochemistry and Biophysics, Vol. 271, No. 1, pp. 56–63
Publisher
Elsevier
Publication Date
May 1989
DOI
10.1016/0003-9861(89)90255-5
ISSN
0003-9861