Journal article
Yeast Recombinase FLP Functions Effectively in Human Cells for Construction of Adenovirus Vectors
Abstract
We have recently developed a high-efficiency method of constructing adenovirus vectors based on Cre-mediated recombination between two plasmids co-transfected into 293 cells. The simplicity and efficiency of this method should greatly expedite the construction of most recombinant vectors. However, this system would not be suitable for constructing vectors bearing loxP sites elsewhere in the genome because of undesirable Cre-mediated vector …
Authors
Ng P; Cummings DT; Evelegh CM; Graham FL
Journal
BioTechniques, Vol. 29, No. 3, pp. 524–528
Publisher
Taylor & Francis
Publication Date
9 2000
DOI
10.2144/00293st04
ISSN
0736-6205