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Two-Step Competitive Hybridization Assay: A Method...
Journal article

Two-Step Competitive Hybridization Assay: A Method for Analyzing Cancer-Related microRNA Embedded in Extracellular Vesicles

Abstract

With an increased understanding of the role of microRNAs (miRNAs) in cancer evolution, there is a growing interest in the use of these non-coding nucleic acids in cancer diagnosis, prognosis, and treatment monitoring. miRNAs embedded in extracellular vesicles (EVs) are of particular interest given that circulating EVs carry cargo that are strongly correlated to their cells of origin such as tumor cells while protecting them from degradation. As …

Authors

Saha S; Allelein S; Pandey R; Medina-Perez P; Osman E; Kuhlmeier D; Soleymani L

Journal

Analytical Chemistry, Vol. 93, No. 48, pp. 15913–15921

Publisher

American Chemical Society (ACS)

Publication Date

December 7, 2021

DOI

10.1021/acs.analchem.1c03165

ISSN

0003-2700