Use of rainbow trout primary epidermal cell cultures as an alternative to immortalized cell lines in toxicity assessment: A study with nonoxynol

Abstract Increased concern has centered around environmental contaminants that exert estrogenic effects. A class of nonionic surfactants, the nonylphenol ethoxylates (such as the compound nonoxynol), are an example of one such group of contaminants. We studied the effects of nonoxynol (α-(4-nonylphenyl)-ω-hydroxypoly-(oxy-1-ethanediyl)) on an epithelial primary culture. The culture system that was employed is a development of an in vitro …