Coculture and transplant of purified CD34+Lin− and CD34−Lin− cells reveals functional interaction between repopulating hematopoietic stem cells

The human hematopoietic stem cell compartment is comprised of repopulating CD34+ and CD34− cells. The interaction between these subsets with respect to their reconstitution capacity in vivo remains to be characterized. Here, lineage-depleted (Lin−) human CD34+ and CD34− hematopoietic cells were isolated from human male and female umbilical cord blood (CB) and transplanted into immune-deficient NOD/SCID EMVnull mice, thereby allowing the use of …