A Syringe‐Based DNAzyme Sensor for Bacterial Detection

Abstract We present a simple approach for bacterial detection that uses a bead‐bound RNA‐cleaving DNAzyme (RcD) and a syringe. The RcD used in this study, F‐EC1, is fluorescently labelled and can be specifically activated by a protein target released specifically by E. coli . Upon immobilization on agarose beads, the kinetic behaviour of F‐EC1 remains similar to that in solution. The F‐EC1‐agarose system was able to detect as few as 1,000 CFUs …