Dose-effect relationship between Ad SIINFEKL-Luc immunization and the induced immune response

Objective: To quantitatively investigate the relationship between a known antigen encoded by adenoviral vector(Ad SIINFEKL-Luc) and the induced immune response. Methods: Murine bone marrow-derived dendritic cells(DC) were infected by Ad SIINFEKL-Luc encoding a fusion protein consisting of chicken ovalbumin MHC class I peptide SIINFEKL(OVAp257-264) and luciferase in different MOI and the expression levels of luciferase(Luc) in DC were …