Journal article
The dependence of the lipolytic activity of Rhizopus arrhizus lipase on surfactant concentration in Aerosol-OT/isooctane reverse micelles and its relationship to enzyme structure
Abstract
Aerosol OT, bis(2-ethylhexyl)sodium sulfosuccinate/isooctane reverse micelles were used to investigate the dependence of the lipolytic activity of Rhizopus arrhizus lipase on surfactant concentration. Kinetic constants for the lipolytic reaction were measured in parallel with structural studies using protein fluorescence and circular dichroism (CD) spectroscopy. Km values remained constant throughout the range of AOT concentrations studied. The …
Authors
Brown ED; Yada RY; Marangoni AG
Journal
Biochimica et Biophysica Acta, Vol. 1161, No. 1, pp. 66–72
Publisher
Elsevier
Publication Date
1 1993
DOI
10.1016/0167-4838(93)90197-y
ISSN
0006-3002