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Estimation of amitriptyline and its metabolites in...
Journal article

Estimation of amitriptyline and its metabolites in serum and urine by GLC using nitrogen-specific detector

Abstract

1. A gas-chromatographic procedure for the estimation of amitriptyline and its metabolites in serum and urine using a nitrogen-specific detector is described. Specially cleaned glassware and purified solvents are used for the extraction of serum to further minimize extraneous peaks. Trimethylamine is added to serum before extraction to improve the recovery of drugs. Urine is refluxed at pH approximately 1 to hydrolyze the conjugates and to …

Authors

Gupta RN; Molnar G; Hill RE; Gupta ML

Journal

Clinical Biochemistry, Vol. 9, No. 5, pp. 247–251

Publisher

Elsevier

Publication Date

1976

DOI

10.1016/s0009-9120(76)80070-7

ISSN

0009-9120