Journal article
A novel and sensitive radioreceptor assay for serum melatonin levels
Abstract
A simple and sensitive radioreceptor assay (RRA) has been developed to measure melatonin levels in serum. The assay is based on competition between 2-[125I]iodomelatonin ([125I]MEL) and melatonin for binding to high-affinity binding sites in chick forebrain. To measure the amount of melatonin present in a serum sample, it was extracted with dichloromethane and added to the assay medium. The percentage inhibition of radioligand binding in the …
Authors
Tenn C; Niles L
Journal
Life Sciences, Vol. 49, No. 25, pp. 1895–1900
Publisher
Elsevier
Publication Date
1 1991
DOI
10.1016/0024-3205(91)90290-r
ISSN
0024-3205