Journal article
Regulatory control of DNA end resection by Sae2 phosphorylation
Abstract
DNA end resection plays a critical function in DNA double-strand break repair pathway choice. Resected DNA ends are refractory to end-joining mechanisms and are instead channeled to homology-directed repair. Using biochemical, genetic, and imaging methods, we show that phosphorylation of Saccharomyces cerevisiae Sae2 controls its capacity to promote the Mre11-Rad50-Xrs2 (MRX) nuclease to initiate resection of blocked DNA ends by at least two …
Authors
Cannavo E; Johnson D; Andres SN; Kissling VM; Reinert JK; Garcia V; Erie DA; Hess D; Thomä NH; Enchev RI
Journal
Nature Communications, Vol. 9, No. 1,
Publisher
Springer Nature
DOI
10.1038/s41467-018-06417-5
ISSN
2041-1723
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Cell CycleDNA Breaks, Double-StrandedDNA End-Joining RepairDNA, FungalDNA-Binding ProteinsEndodeoxyribonucleasesEndonucleasesExodeoxyribonucleasesMeiosisMultiprotein ComplexesPhosphorylationProtein BindingProtein MultimerizationRecombinational DNA RepairSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins