Journal article
Chromogenic Carbamate and Acetal Substrates for Glycosaminidases
Abstract
The enzyme Dispersin B is a medicinally relevant β-hexosaminidase with potential for use in the treatment of biofilm-related infections. Here we describe the synthesis and evaluation of a p-nitrophenyl glycosyl carbamate and a p-nitrophenyl glycosyl acetal as improved substrates of Dispersin B. The p-nitrophenyl glycosyl carbamate shows substantially improved analytical signal in continuous assays and superior kinetics (kcat/Km 6.1 mM−1 s−1) …
Authors
Chibba A; Dasgupta S; Yakandawala N; Madhyastha S; Nitz M
Journal
Journal of Carbohydrate Chemistry, Vol. 30, No. 7-9, pp. 549–558
Publisher
Taylor & Francis
Publication Date
September 1, 2011
DOI
10.1080/07328303.2011.610543
ISSN
0732-8303