Journal article
Decreased epithelial barrier function evoked by exposure to metabolic stress and nonpathogenic E. coli is enhanced by TNF-α
Abstract
A defect in mitochondrial activity contributes to many diseases. We have shown that monolayers of the human colonic T84 epithelial cell line exposed to dinitrophenol (DNP, uncouples oxidative phosphorylation) and nonpathogenic Escherichia coli (E. coli) (strain HB101) display decreased barrier function. Here the impact of DNP on macrophage activity and the effect of TNF-alpha, DNP, and E. coli on epithelial permeability were assessed. DNP …
Authors
Lewis K; Caldwell J; Phan V; Prescott D; Nazli A; Wang A; Soderhölm JD; Perdue MH; Sherman PM; McKay DM
Journal
AJP Gastrointestinal and Liver Physiology, Vol. 294, No. 3, pp. g669–g678
Publisher
American Physiological Society
Publication Date
3 2008
DOI
10.1152/ajpgi.00382.2007
ISSN
0193-1857
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Adenosine TriphosphateBlotting, WesternCaspasesCell DeathCell LineCells, CulturedDinitrophenolsElectric ImpedanceEpitheliumEscherichia coli InfectionsFeedback, PhysiologicalHumansInterleukin-1betaInterleukin-6LeupeptinsLipopolysaccharidesMacrophagesNF-kappa BNitrilesPermeabilityStress, PhysiologicalSulfonesTetrazolium SaltsThiazolesTumor Necrosis Factor-alphaUncoupling Agents