Journal article
Characterization of an RNA-Cleaving Deoxyribozyme with Optimal Activity at pH 5
Abstract
An in vitro selection endeavor previously executed by our laboratory led to the isolation of a set of RNA-cleaving deoxyribozymes that thrive under acidic conditions [Liu, Z., Mei, S. H., Brennan, J. D., and Li, Y. (2003) J. Am. Chem. Soc. 125, 7539-7545]. One of these sequences, coined pH5DZ1, is a 100-nucleotide (nt) cis-acting enzyme that was found to exhibit high cleavage activity near pH 5. Herein, we seek to deduce the properties and …
Authors
Kandadai SA; Mok WWK; Ali M; Li Y
Journal
Biochemistry, Vol. 48, No. 31, pp. 7383–7391
Publisher
American Chemical Society (ACS)
Publication Date
August 11, 2009
DOI
10.1021/bi900631u
ISSN
0006-2960