Journal article
Gi Activator Region of α2A-Adrenergic Receptors: Distinct Basic Residues Mediate Gi versus Gs Activation
Abstract
The structural determinants of G protein coupling versus activation by G protein-coupled receptors are not well understood. We examine the role of two distinct basic regions in the carboxyl terminal portion of the third intracellular loop of the alpha(2A)-adrenergic receptor to dissect these aspects of function. Changing three arginines to alanines by mutagenesis and stable expression in Chinese hamster ovary-K1 cells impaired the …
Authors
Wade SM; Lim WK; Lan K-L; Chung DA; Nanamori M; Neubig RR
Journal
Molecular Pharmacology, Vol. 56, No. 5, pp. 1005–1013
Publisher
Elsevier
Publication Date
November 1, 1999
DOI
10.1124/mol.56.5.1005
ISSN
0026-895X