Journal article
Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
Abstract
Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in Clostridium pasteurianum for markerless genome editing. Since 74% of species harbor CRISPR-Cas loci in Clostridium, we also explored the prospect of co-opting host-encoded CRISPR-Cas machinery for genome editing. Motivation for this work was bolstered from the …
Authors
Pyne ME; Bruder MR; Moo-Young M; Chung DA; Chou CP
Journal
Scientific Reports, Vol. 6, No. 1,
Publisher
Springer Nature
DOI
10.1038/srep25666
ISSN
2045-2322