Separation of human albumin and IgG by a membrane-based integrated bioseparation technique involving simultaneous precipitation, microfiltration and membrane adsorption

This paper discusses an integrated one-step bioseparation technique for separation of human plasma proteins HSA and HIgG. This technique which was carried out in the pulse-input mode combined three separation processes, i.e. (a) in-situ ammonium sulfate induced precipitation, (b) microfiltration, and (c) membrane adsorption, all of which were carried out simultaneously within the same membrane filtration device. The microfiltration membrane …