A novel approach for high-resolution protein–protein separation by ultrafiltration using a dual-facilitating agent

This paper discusses a novel approach based on the use of the protein BSA as a dual-facilitating agent for manipulating the separation of two model proteins having comparable molecular weights. When a binary mixture of lysozyme and myoglobin alone was ultrafiltered, lysozyme was transmitted through the membrane to a substantially greater extent than myoglobin. However, when BSA was added to the feed solution, lysozyme was largely retained by …