Towards a homogeneous fluorescence assay for a herbicide: characterization of the interactions of fusilade, bromomethylmethoxy coumarin derivatized fusilade, and anti-fusilade IgG antibody

The hapten 2-(4-(5-trifluoromethyl-2-pyridyloxy)phenoxy)-propionic acid (fusilade), was derivatized with the fluorescent probe 4-bromomethyl-7-methoxycoumarin (BrMmC). The labelled hapten (fusilade-coumarin, F-C) was incubated with antifusilade IgG and the fluorescence spectra, intensity and time resolved fluorescence decay of the coumarin derivative were measured to study the complexation process of the hapten-antibody system. The fluorescence …