Ultraviolet transcriptional unit mapping for the late genes in adenovirus type 2

Human KB cells lytically infected with human adenovirus type 2 were pulse labeled with [35S]methionine during the late period of viral infection, and the resulting labeled polypeptides were analyzed by SDS-polyacrylamide-gel electrophoresis and autoradiography. A differential reduction of late adenovirus type 2 protein synthesis was observed following uv irradiation of the infected cells. The uv inactivation of the synthesis of specific viral proteins was assumed to correlate with physical distance between the genes encoding these proteins and their promotors. In this way the relative positions of several late adenovirus type 2 gene locations were determined within each of two transcription units.