Plasmid DNA transmission through charged ultrafiltration membranes

Ultrafiltration can potentially be used for the concentration and purification of plasmid DNA. The objective of this study was to determine the role of electrostatic interactions on the transmission of a supercoiled plasmid through negatively charged composite regenerated cellulose membranes produced by covalent attachment of sulphonic acid groups to the base cellulose. The plasmid sieving coefficient at any given flux decreased with increasing …