Characterization, multiplex conditions, and cross‐species utility of tetranucleotide microsatellite loci for Blanchard's cricket frog (Acris crepitans blanchardi)

Abstract We have isolated and characterized 17 tetranucleotide microsatellite loci for Blanchard's cricket frog ( Acris crepitans blanchardi ), an anuran common in the central USA. Sixteen loci were organized into four multiplex amplification reactions. These loci were highly polymorphic when screened in 55 individuals from two distant populations, with 11–48 alleles per locus (average = 24.8). Observed and expected heterozygosities ranged …