Adhesion to some extracellular matrix components of heat-treated HTC hepatoma cells

HTC hepatoma cell cultures were submitted to hyperthermic treatment at 42°C or 44°C for one hour. They were tested at 0 and 12 hrs after treatment to determine the number of recovered vital cells, their ability to produce clones and to adhere to plastic surfaces previously coated with laminin, collagen type IV and fibronectin. Only the treatment carried out at 44°C proved to decrease the number of the recovered vital cells and their clonal efficiency. The adhesion of these cells to laminin decreases after the treatment at 42°C or 44°C. The adhesion to collagen type IV increases only in the cells at 12 hrs after the treatment at 42°C or 44°C. The adhesion to fibronectin slighty decreases only at 12 hrs after hyperthermia at 42°C.