Journal article
Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology
Abstract
MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New ‘next-generation’ technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for …
Authors
Morin RD; Zhao Y; Prabhu A-L; Dhalla N; McDonald H; Pandoh P; Tam A; Zeng T; Hirst M; Marra M
Journal
Methods in Molecular Biology, Vol. 650, , pp. 173–199
Publisher
Springer Nature
Publication Date
2010
DOI
10.1007/978-1-60761-769-3_14
ISSN
1064-3745