Journal article
Purification and characterization of a sperm motility‐dynein ATPase inhibitor from boar seminal plasma
Abstract
A sperm motility inhibitor from boar seminal plasma was purified. The purification procedure included dialysis against 0.1 M Tris-HCl containing 0.1 mM DTT and chromatographies on SP-Sephadex C-25 and Phenyl-Sepharose CL-4B. With this procedure, the seminal plasma motility inhibitor (SPMI) preparation was highly purified with a 18% recovery of inhibitory activity. The molecular weight of SPMI in native conditions has been estimated at 50,000 by …
Authors
Iwamoto T; Tsang A; Luterman M; Dickson J; de Lamirande E; Gagnon C; Okuno M; Mohri H
Journal
Molecular Reproduction and Development, Vol. 31, No. 1, pp. 55–62
Publisher
Wiley
Publication Date
1 1992
DOI
10.1002/mrd.1080310110
ISSN
1040-452X