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Measurement of D2O Concentrations at Tracer Levels...
Journal article

Measurement of D2O Concentrations at Tracer Levels in Small Samples Obtained from Paediatric Patients

Abstract

A micro-method is described for the determination of trace amounts of D2O in serum, saliva and urine, requiring 100 microliters of sample. H2O/D2O are rapidly isolated from serum, saliva or urine by vacuum distillation. D2O concentrations in H2O are detected by infrared spectroscopy utilizing the integrated absorption of the OD-bond at 2510 cm-1 in the range of 2675 to 2460 cm-1. Calibration is done using standard solutions of H2O/D2O. The …

Authors

Fusch C; Moeller H

Journal

Clinical Chemistry and Laboratory Medicine, Vol. 26, No. 11, pp. 715–722

Publisher

De Gruyter

Publication Date

November 1988

DOI

10.1515/cclm.1988.26.11.715

ISSN

1434-6621