Journal article
Column liquid chromatographic determination of bupivacaine in human serum using solid-phase extraction
Abstract
A convenient and economical procedure for the extraction of bupivacaine and its major metabolites, desbutylbupivacaine and 4'-hydroxybupivacaine, from serum is described. A 0.25-ml aliquot of 4 micrograms/ml solution of N-pentyl-2,6-pipecoloxylidide used as the internal standard and an equal volume of the serum sample are applied to a 1-ml BondElut C18 silica extraction column which has been conditioned by subsequent washing once with 1 M HCl, …
Authors
Gupta RN; Dauphin A
Journal
Journal of Chromatography B, Vol. 658, No. 1, pp. 113–119
Publisher
Elsevier
Publication Date
August 1994
DOI
10.1016/0378-4347(94)00214-2
ISSN
1570-0232