Journal article
Human Serum Proteins Preseparated by Electrophoresis or Chromatography Followed by Tandem Mass Spectrometry
Abstract
Electrophoretic and chromatographic sample preparations were compared and together detected the presence of some 600 types of protein products in human serum. Proteins from crude serum preseparated by ionic electrophoresis, chromatography, or a combination of both were analyzed. Proteins were digested with trypsin or chymotrypsin. Naturally occurring peptides were also collected by reversed-phase chromatography. The resulting peptides were …
Authors
Marshall J; Jankowski A; Furesz S; Kireeva I; Barker L; Dombrovsky M; Zhu W; Jacks K; Ingratta L; Bruin J
Journal
Journal of Proteome Research, Vol. 3, No. 3, pp. 364–382
Publisher
American Chemical Society (ACS)
Publication Date
June 1, 2004
DOI
10.1021/pr034039p
ISSN
1535-3893