Journal article
Purification of humanized monoclonal antibody by hydrophobic interaction membrane chromatography
Abstract
Humanized monoclonal antibodies (mAbs) hold significant promise as biopharmaceuticals. One of the main challenges faced in the purification of mAbs is their separation from bovine serum albumin, which is the main protein present in most mammalian cell culture media. This paper discusses the purification of humanized mAb hIgG1-CD4 from CHO cell culture media by hydrophobic interaction membrane chromatography using a stack of microporous …
Authors
Ghosh R; Wang L
Journal
Journal of Chromatography A, Vol. 1107, No. 1-2, pp. 104–109
Publisher
Elsevier
Publication Date
2 2006
DOI
10.1016/j.chroma.2005.12.035
ISSN
0021-9673