A Pulsed Tangential-Flow Ultrafiltration Technique for Studying Protein-Drug Binding

We describe a pulsed tangential-flow ultrafiltration technique for rapid analysis of protein-drug binding. A protein-drug pulse was injected into a tangential-flow membrane device and made to flow parallel to the surface of a protein-retaining ultrafiltration membrane. The protein and protein-drug complexes were flushed out of the device in the retentate stream, whereas the free drug present in the permeate stream was quantified using on-line …