Purification of chimeric heavy chain monoclonal antibody EG2‐hFc using hydrophobic interaction membrane chromatography: An alternative to protein‐A affinity chromatography

Heavy chain monoclonal antibodies are being considered as alternative to whole-IgG monoclonal antibodies for certain niche applications. Protein-A chromatography which is widely used for purifying IgG monoclonal antibodies is also used for purifying heavy chain monoclonal antibodies as these molecules possess fully functional Fc regions. However, the acidic conditions used to elute bound antibody may sometimes also leach protein-A, which is …