Journal article
Sensitive solid-phase immune electron microscopy double-antibody technique with gold-immunoglobulin G complexes for detecting rotavirus in cell culture and feces
Abstract
A new solid-phase immune electron microscopy double-antibody colloidal-gold technique (SPIEMDAGT) was developed and compared with direct electron microscopy, direct immune electron microscopy, and enzyme immunoassay for detecting rotavirus. Guinea pig and rabbit antirotavirus antisera were used as capture and detector antibodies, respectively, and goat anti-rabbit immunoglobulin G-gold complexes were employed as a label. Animal rotavirus in …
Authors
Wu B; Mahony JB; Simon G; Chernesky MA
Journal
Journal of Clinical Microbiology, Vol. 28, No. 5, pp. 864–868
Publisher
American Society for Microbiology
Publication Date
May 1990
DOI
10.1128/jcm.28.5.864-868.1990
ISSN
0095-1137