Journal article
Studies on canine gastric antrum smooth muscle: preparation and characterization of a plasma membrane enriched fraction
Abstract
A method is described for preparation of large amounts of a plasma membrane (PM) enriched fraction from the smooth muscle of dog antrum. It consists of preparing microsomes, treating them with ATP + EGTA + Mg, centrifuging in 30% sucrose and then centrifuging the resulting supernatant in 15% sucrose to yield the plasma membrane enriched fraction P6. The subcellular fractions obtained at various steps during purification were characterized by: …
Authors
Grover AK; Oakes P; Sipos SN; Kwan CY; Garfield RE
Journal
Canadian Journal of Physiology and Pharmacology, Vol. 61, No. 8, pp. 927–940
Publisher
Canadian Science Publishing
Publication Date
August 1, 1983
DOI
10.1139/y83-139
ISSN
0008-4212