Journal article
Determination of immunoreactive melatonin in the colon of the rat by immunocytochemistry.
Abstract
Melatonin immunofluorescence in the rat colon was measured with a cadmium sulfide photometer in conjunction with an immunohistological double antibody procedure. The method proved to be very reliable with 5-10% intra- and interassay reliabilities. In addition, manipulation of the primary antibody concentration, the incubation time, and the nature of the first antibody produced predicted changes in fluorescence intensity. Treatment with …
Authors
Holloway WR; Grota LJ; Brown GM
Journal
Journal of Histochemistry & Cytochemistry, Vol. 28, No. 3, pp. 255–262
Publisher
SAGE Publications
Publication Date
March 1980
DOI
10.1177/28.3.6444434
ISSN
0022-1554