Effect of disulphonic stilbenes on Ca2+ transport in smooth muscle plasma membranes Journal Articles uri icon

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abstract

  • We studied the effects of two disulphonic stilbenes, 4′,4′-diisothiocyano-2,2′-stilbene disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyano-2,2′-stilbene disulphonic acid (SITS), on Ca2+ transport by plasma membrane vesicles from the circular muscle of the dog stomach. Both compounds inhibited ATP-dependent Ca2+ uptake and reduce the leak from loaded vesicles. The inhibition produced could not be significantly reduced by either permeant anions or by increasing the level of free Ca2+. The effects of DIDS could be rendered irreversible by incubating the membranes with this agent at 37 °C.

publication date

  • September 1, 1984