Journal article
Entrapment of Fluorescent Signaling DNA Aptamers in Sol−Gel-Derived Silica
Abstract
We report on the first successful immobilization of a DNA aptamer, in particular, a fluorescence-signaling DNA aptamer, within a sol-gel-derived matrix. The specific aptamer examined in this study undergoes a structural switch in the presence of adenosine triphosphate (ATP) to release a dabcyl-labeled nucleotide strand (QDNA), which in turn relieves the quenching of a fluorescein label that is also present in the aptamer structure. It was …
Authors
Rupcich N; Nutiu R; Li Y; Brennan JD
Journal
Analytical Chemistry, Vol. 77, No. 14, pp. 4300–4307
Publisher
American Chemical Society (ACS)
Publication Date
July 1, 2005
DOI
10.1021/ac0506480
ISSN
0003-2700