Journal article
Evaluation of the Calmodulin‐SOX9 Interaction by “Magnetic Fishing” Coupled to Mass Spectrometry
Abstract
Disruption of calmodulin (CaM)-based protein interactions has been touted as a potential means for modulating several disease pathways. Among these is SOX9, which is a DNA binding protein that is involved in chrondrocyte differentiation and regulation of the hormones that control sexual development. In this work, we employed a "magnetic fishing"/mass spectrometry assay in conjunction with intrinsic fluorescence to examine the interaction of CaM …
Authors
McFadden MJ; Hryciw T; Brown A; Junop MS; Brennan JD
Journal
ChemBioChem, Vol. 15, No. 16, pp. 2411–2419
Publisher
Wiley
Publication Date
November 3, 2014
DOI
10.1002/cbic.201402414
ISSN
1439-4227