Journal article
Transient Expression of Wild-Type or Biologically Inactive Telomerase Allows the Formation of Artificial Telomeres in Mortal Human Cells
Abstract
Telomere seeding, the formation of artificial telomeres, has been routinely successful in immortalized but not normal human cells. We compared seeding efficiencies in preimmortal and immortal SV40-transformed cells using plasmid telomeres with T(2)AG(3) tracts of 1600 and 3200 bp. Seeding occurred only in immortal cells, indicating that transformed preimmortal cells behave like normal cells vis à vis formation of new telomeres and that …
Authors
Guiducci C; Anglana M; Wang A; Bacchetti S
Journal
Experimental Cell Research, Vol. 265, No. 2, pp. 304–311
Publisher
Elsevier
Publication Date
May 2001
DOI
10.1006/excr.2001.5189
ISSN
0014-4827