Journal article
Extraction Efficiency of an Extraction Buffer Used to Quantify Lysozyme Deposition on Conventional and Silicone Hydrogel Contact Lens Materials
Abstract
PURPOSE: Extracting lysozyme from Food and Drug Administration group IV etafilcon lenses by using 0.2% trifluoroacetic acid and acetonitrile (TFA/ACN) is a well-established procedure. TFA/ACN has been the extraction buffer of choice for extracting proteins from silicone hydrogel contact lenses. The purpose of this study was to determine the efficiency of TFA/ACN in extracting lysozyme from silicone hydrogel and etafilcon lenses by using an in …
Authors
Subbaraman LN; Glasier MA; Senchyna M; Sheardown H; Jones L
Journal
Eye & Contact Lens Science & Clinical Practice, Vol. 33, No. 4, pp. 169–173
Publisher
Wolters Kluwer
Publication Date
July 2007
DOI
10.1097/01.icl.0000248155.23800.20
ISSN
1542-2321