Concerning the presence of the cytokinin, N6-(Δ2-isopentnyl) adenine, in cultures of Corynebacterium fascians

Corynebacterium fascians causes a fasciation disease in a number of dicotyledons and this disease appears to be caused by compounds with cytokinin activity elaborated by the infecting bacteria. Extractions of C. fascians in late logarithmic phase under conditions where the pH never falls below 7.0 yield about 2 μg/l of N6-(Δ2, a potent cytokinin. If a mild acidification step is included in the extraction procedure the yield increases to about …