Journal article
Quantitative estimation of tryptophan in plasma and urine using thin-layer chromatography (TLC) and induced fluorescence
Abstract
A new method for the determination of free tryptophan in plasma and urine is described. After isolation of tryptophan by thin-layer chromatography, a photometric measurement of induced ultraviolet fluorescence is used for its quantitative estimation. The intensity of induced fluorescence in ultraviolet light is directly proportional to the amount of tryptophan present. This method has advantages for it does not require previous precipitation of …
Authors
Gibbs CCJ; Saunders SJ; Sweeney GD
Journal
Clinica Chimica Acta, Vol. 17, No. 3, pp. 317–323
Publisher
Elsevier
Publication Date
September 1967
DOI
10.1016/0009-8981(67)90204-5
ISSN
0009-8981