Journal article
Quantitation of urinary porphyrins by use of second-derivative spectroscopy.
Abstract
We describe a simple, rapid procedure for measuring porphyrins in urine. After acidification of a urine specimen with hydrochloric acid, the second derivative of the absorption spectrum is recorded in the region of the Soret band maximum. The amplitude of the deflection is linearly related to porphyrin concentration, and the point at which the second derivative minimum occurs provides an estimate of the ratio of uroporphyrin to coproporphyrin …
Authors
Jones KG; Sweeney GD
Journal
Clinical Chemistry, Vol. 25, No. 1, pp. 71–74
Publisher
Oxford University Press (OUP)
Publication Date
January 1, 1979
DOI
10.1093/clinchem/25.1.71
ISSN
0009-9147