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Interaction of human lactoferrin with the rat...
Journal article

Interaction of human lactoferrin with the rat liver

Abstract

Binding of human lactoferrin (hLf) by purified rat liver plasma membranes was studied to clarify whether the liver possesses specific hLf receptors. The binding was rapid between 4 degrees and 37 degrees C, with a pH optimum close to 5.0. At 22 degrees C and in glycine-NaOH (5 mM, pH 7.4) containing 150 mM NaCl and 0.5% albumin, 1 microgram of membrane bound a maximum of 11.8 ng hLf. The dissociation constant of the interaction was 1.6 X 10(-7) …

Authors

Debanne MT; Regoeczi E; Sweeney GD; Krestynski F

Journal

American Journal of Physiology, Vol. 248, No. 4, pp. g463–g469

Publisher

American Physiological Society

Publication Date

April 1, 1985

DOI

10.1152/ajpgi.1985.248.4.g463

ISSN

0002-9513