Journal article
The affinity of human, rabbit and bovine thrombins for sepharose-lysine and other conjugates
Abstract
Human, rabbit and bovine thrombins are shown to possess marked affinities for Sepharose-lysine. Using either Xa-activated crude prothrombins (human, rabbit) or a commercial thrombin sample (bovine), the enzyme was isolated in a single chromatographic step by the affinity medium and preparations of high specific activity were obtained. The relevance of bound-lysine for the affinity of the thrombins was studied using other Sepharose conjugates …
Authors
Hatton MWC; Regoeczi E
Journal
Biochimica et Biophysica Acta, Vol. 427, No. 2, pp. 575–585
Publisher
Elsevier
Publication Date
April 1976
DOI
10.1016/0005-2795(76)90200-2
ISSN
0006-3002