Journal article
Isolation and partial characterization of rabbit plasma α1-antitrypsin
Abstract
Alpha1-Antitrypsin was isolated from rabbit plasma by salting out with (NH4)2SO4 followed by ion-exchange chromatography either on DEAE-Sephadex or DEAE-cellulose (each at pH8.8 and 6.5), and affinity chromatography on Sepharose-Cibacron Blue and Sepharose-concanavalin A. The protein thus obtained was homogeneous during crossed immunoelectrophoresis by using an antiserum to whole rabbit plasma, but it migrated as two broad bands when …
Authors
Koj A; Hatton MW; Wong KL; Regoeczi E
Journal
Biochemical Journal, Vol. 169, No. 3, pp. 589–596
Publisher
Portland Press
Publication Date
March 1, 1978
DOI
10.1042/bj1690589
ISSN
0264-6021