Localization of the Oxytocin Receptor in the Plasma Membrane of Rat Myometrium Journal Articles

abstract

• The distribution of [3H]oxytocin binding sites among various subcellular fractions of rat myometrium paralleled the distribution of 5′‐nucleotidase, a plasma membrane marker enzyme, but not of NADPH‐cytochrome c reductase or succinate‐cytochrome c reductase, which are endoplasmic reticulum and mitochondrial marker enzymes respectively. [3H]Oxytocin binding to the most enriched plasma membrane fraction showed the degree of selectivity with respect to hormone analogues that is expected for the oxytocin receptor. The binding of oxytocin to this fraction showed an apparent Kd of 1.98 × 10−9 M and a capacity of 1.28 pmol mg−1. It is concluded that the oxytocin receptor is located on the plasma membrane of the smooth muscle cells of the rat uterus.

authors

• Crankshaw, Denis James
• BRANDA, Luis A
• MATLIB, Mohammed A
• DANIEL, Edwin E

status

• published 

publication date

• May 1978

has subject area

• 0304 Medicinal and Biomolecular Chemistry (FoR)
• 0601 Biochemistry and Cell Biology (FoR)
• 1101 Medical Biochemistry and Metabolomics (FoR)
• Animals (MeSH)
• Biochemistry & Molecular Biology (Science Metrix)
• Cell Fractionation (MeSH)
• Cell Membrane (MeSH)
• Cytochrome Reductases (MeSH)
• Female (MeSH)
• Myometrium (MeSH)
• Nucleotidases (MeSH)
• Oxytocin (MeSH)
• Rats (MeSH)
• Receptors, Cell Surface (MeSH)
• Subcellular Fractions (MeSH)
• Uterus (MeSH)

published in

• The FEBS Journal  Journal

keywords

• Animals
• Cell Fractionation
• Cell Membrane
• Cytochrome Reductases
• Female
• Myometrium
• Nucleotidases
• Oxytocin
• Rats
• Receptors, Cell Surface
• Subcellular Fractions
• Uterus

Digital Object Identifier (DOI)

• 10.1111/j.1432-1033.1978.tb12331.x

PubMed ID

• 207528

start page

• 481

end page

• 486

volume

• 86

issue

• 2