Journal article
Prolonged in vivo anticoagulant activity of a hirudin–albumin fusion protein secreted from Pichia pastoris
Abstract
Hirudin is a small, proteinaceous thrombin inhibitor that clears rapidly from the circulation. A hexahistidine-tagged hirudin-rabbit serum albumin (RSA) fusion protein, HLAH6, was characterized following secretion from Pichia pastoris. HLAH6 bound to immobilized nickel, anti-RSA, and anti-hexahistidine antibodies, and contained the expected (ITYTD) N-terminus. Its spectrometric mass was 74,490 (versus the theoretical mass of 74,410 and sodium …
Authors
Sheffield WP; Smith IJ; Syed S; Bhakta V
Journal
Blood Coagulation & Fibrinolysis, Vol. 12, No. 6, pp. 433–443
Publisher
Wolters Kluwer
Publication Date
September 2001
DOI
10.1097/00001721-200109000-00003
ISSN
0957-5235