Journal article
Expression, refolding, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgE
Abstract
AlgE is an outer membrane protein present in alginate-producing (mucoid) Pseudomonas aeruginosa. AlgE has been overexpressed in insoluble inclusion bodies, purified under denaturing conditions and refolded in a buffer containing decyl beta-D-maltopyranoside. Purified refolded AlgE was detergent-exchanged into n-octyl tetraoxyethylene and diffraction-quality crystals were grown using the hanging-drop vapor-diffusion method. The crystals grew as …
Authors
Whitney JCC; Neculai AM; Ohman DE; Howell PL
Journal
Acta Crystallographica Section F: Structural Biology Communications, Vol. 65, No. 5, pp. 463–466
Publisher
International Union of Crystallography (IUCr)
Publication Date
May 1, 2009
DOI
10.1107/s174430910901094x
ISSN
2053-230X